Horticultural Science Department at UF

Tomato has notoriously low levels of intraspecific sequence variation and it is difficult to identify polymorphic markers.  Tomato populations derived from elite parents segregate for a large number of phenotypic characteristics.  However, the lack of polymorphic markers impedes the ability to map traits in these genetic backgrounds.  To overcome the hurdle of marker discovery, a re-sequencing technique will be applied for single nucleotide polymorphism (SNP) discovery in tomato targeting genomic sequences with known genetic map positions. The approach uses microarray-based genomic selection (MGS) for the enrichment of targeted genomic sequences in tomato, combined with next-generation sequencing (i.e., 454-FLX and/or SOLID) of the selected targets.  Eight elite Florida tomato varieties and breeding lines will be used in the experiment including one near isogenic line with TYLCV resistance to potentially identify markers closely linked to the resistance gene.

For more on Dr. Jeremy Edwards Research
Edwards' Publications